勁馬產(chǎn)品文獻:小鼠BrdU,TNF-α ELISA試劑盒引用文獻

【文獻標題】Tissue factor pathway inhibitor gene transfer prevents vascular smooth muscle cell proliferation by interfering with the MCP-3/CCR2 pathway

【作者】Yu Fu, Dandan Ma, Yue Liu,et.al

【作者單位】哈爾濱醫(yī)科大學( Harbin Medical University)

【文獻中引用產(chǎn)品】

小鼠溴脫氧核苷(BrdU)ELISA試劑盒

小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

【DOI】doi:10.1038/labinvest.2015.106

【影響因子(IF)】6.7

【出版期刊】《Laboratory Investigation》

【產(chǎn)品原文引用】

Cell Proliferation StudiesVSMC proliferation was analyzed via measuring DNA synthesis with a colorimetric bromodeoxyuridine (BrdU) ELISA kit according to the manufacturer’s instructions (Shanghai JinMa Laboratory and Equipment Company,Shanghai, China). Briefly, VSMCs were seeded in 24-well plates at a density of 5 × 104 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, after which the cell culture supernatants were collected and centrifuged at 12 000 g for 15 min at 4 °C; the extracted supernatants were immediately frozen at ? 80 °C until use. Absorbance values at 450 nm were determined using an ELISA reader (Bio-Rad, CA, USA).

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