【文獻(xiàn)標(biāo)題】Anti-Inflammatory Activity of Water-Soluble Polysaccharide of Agaricus blazei Murill on Ovariectomized Osteopenic Rats

【作者】Peng Wang, Xiao-Tao Li, Lei Sun et.al

【作者單位】 Harbin Medical University（哈爾濱醫(yī)科大學(xué)）

【文獻(xiàn)中引用產(chǎn)品】

大鼠白介素1β (IL-1β)ELISA試劑盒

大鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

【關(guān)鍵詞】無

【DOI】dx.doi.org/10.1155/2013/164817

【影響因子(IF)】2.6

【出版期刊】《Evidence-Based Complementary and Alternative Medicine》

【產(chǎn)品原文引用】

Estimation of Serum IL-1β and TNF-α Level. Serum samples were disintegrated in 5 volumes of ice-cold RIPA buffer. After incubation on ice for 30 minutes, samples were centrifuged twice at 20,000 Xg for 15 minutes at 4°C. The resulting supernatants were used for assay. The concentration of IL-1β and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.

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