【文獻標題】iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae

【作者】Rining Zhu, Chuntong Baoet.al

【作者單位】吉林大學（ Jilin University）

【文獻中引用產(chǎn)品】

豬甘露糖受體C1樣蛋白1(MRC1)ELISA試劑盒

豬過氧化物酶2（PRDX2）ELISA試劑盒

豬補體因子B(CFB)ELISA

豬整合素β（ITG-β）ELISA試劑盒

豬E鈣粘附分子(E-Cadherin)ELISA試劑盒

【關鍵詞】A. pleuropneumoniae, iTRAQ, Porcine pleuropneumonia, Comparative proteomics, Serum

【DOI】doi.org/10.1186/s13568-020-01057-9

【影響因子(IF)】4.12

【出版期刊】《AMB Expr》

【產(chǎn)品原文引用】Five proteins were selected for validation by ELISA:MRC1,PRDX2,CFB,ITGB and E-Cadherin. Te serum samples were allowed to clot for 1h at room temperature and then separated by centrifugation at 2000×g for 

15min at 4?°C. Te serum was then frozen until analyzed by ELISA. Te concentration of MRC1,PRDX2,CFB,ITGB and E-Cadherin was determined using commercial porcine ELISA kits (Shanghai Jinma Biological Technology, Inc., China) according to the manufacturer’s instructions. All samples were analyzed in duplicate. Cytokine concentrations were calculated using linear regression analysis based on the OD of the cytokine standards provided by the manufacturer.

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