【文獻(xiàn)標(biāo)題】Vitamin E-Inhibited Phoxim-Induced Renal Oxidative Stress and Mitochondrial Apoptosis In Vivo and In Vitro of Piglets

【作者】Jing Zhang, Yuecheng Sun, Wentao Songet.al

【作者單位】Northeast Agricultural University（東北農(nóng)業(yè)大學(xué)）

【文獻(xiàn)中引用產(chǎn)品】

豬丙二醛（MDA）ELISA試劑盒

豬總抗氧化能力（T-AOC）檢測(cè)試劑盒

豬超氧化物歧化酶(SOD)ELISA試劑盒

豬過氧化氫酶（CAT）ELISA試劑盒

豬谷胱-甘肽過氧化物酶（GSH-Px）ELISA試劑盒

豬細(xì)胞-色素C氧化酶ELISA試劑盒

【關(guān)鍵詞】phoxim; vitamin E; oxidative stress; mitochondrion; apoptosis

【DOI】doi.org/10.3390/antiox12112000

【影響因子(IF)】5.9

【出版期刊】《antioxidants》

【產(chǎn)品原文引用】

Oxidation–Antioxidant Parameters

In vivo, the serum ROS levels were determined in the piglets by an enzyme-linkedimmunosorbent assay (ELISA), which was operated strictly according to the instructions of the kit (Shanghai Jinma Co., Ltd., Shanghai, China) on a Labsystems Multiskan MS (Finland). The antioxidant enzyme parameters were tested using an ultraviolet spectrophotometer (UV-2410PC model, Shimadzu Corp., Kyoto, Japan) according to the instructions of the commercial diagnostics kit (Nanjing Jiancheng Biotechnology Co., Ltd., Nanjing, China).

The parameters that were evaluated in the kidney were malondialdehyde (MDA), total antioxidant capacity (T-AOC),superoxide dismutase (SOD), glutathione (GSH), catalase(CAT), and glutathione peroxidase (GSH-Px).

Mitochondrial Function

For mitochondrial function, the activities of succinate dehydrogenase (SDH), cytochrome c oxidase (COX), and Ca2+-Mg2+-ATPase in the PK15 cells were determined by ELISA. The enzyme activity was detected by a Labsystems Multiskan MS (Finland) system in strict accordance with the instructions of the kit (Shanghai Jinma Co., Ltd.,Shanghai, China).

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